1、Users组用户对站点文件没有读写权限 解决办法：给站点目录设置users组读写权限
2、站点Asp.net版本选择不正确 这种问题会出现在多版本FrameWork共存的情况下，如Asp.net1.1和2.0，详细请看http://bbs.51aspx.com/showtopic-750.html 解决办法：站点-》属性-》选择当前的Asp.net版本
3、应用程序池冲突 解决办法：在应用程序池-》新建应用程序池，然后在站点-》属性-》主目录 选择刚才建立的应用程序池
本人测试第三个方法好用。
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